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levonyuk_marina@mail.ru

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. , . .. 109 151, ( ) ( ) 1 RIG0 . 10 - 1 INRA ().

- [10, 11] [12]. SSR- , , [1]. STS- orfH522, 1 [3]. HRG02, Rf1, 1, SCAR- OPY10, . [4]. 1,8-2 % .

. 109 151 , STS- orfH522 1 RIG0 (. 1). , - - .

VI , , 20 11 .

1 orfH 1-3 109 ; 4-6 109 1; 7-9 109 RIG0; 10-12 151 ;

13-15 151 1; 16-18 151 RIG0; 109 151 8- SSR- (. 2).

2 SSR- 2 % : 1, 7, 13, 19, 25, 31, 37, 43 109 ; 2, 8, 14, 20, 26, 32, 38, 44 109 1;

3, 9, 15, 21, 27, 33, 39, 45 - 109 RIG0; 4, 10, 16, 22, 28, 34, 40, 46 151 ; 5, 11, 17, 23, 29, 35, 41, 47 151 1; 6, 12, 18, 24, 30, 36, 42, 48 - 151 RIG0.

: 1-6 - ORS 6; 7-12 ORS 509; 13-18 Ha 432; 19-24 - 514; 25-30 1442;

31-36 - 1608; 37-42 IUB 4; 43-48 IUB HRG02/OPY10 Rf1, 1, VI , , 2011 .

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14- , Rf1 HRG02/OPY10 631, 637, 755, 766, 771, 792, 793 900, RIL 38, RIL 100, RIL 130, RIL 228 (. 3). 160 365. , - F1 : 365 637. 365, Rf1, 1.

, , 365 . 637, , . Rf1 . , HRG02/OPY10 Rf1, , .

3 HRG 1,8 % 1 - 792; 2 793; 3 RIL 38; 4, 5 RIL 228; 6, 7 RIL 130; 8 637;

9, 10 365; 11 - 160; 12, 13 900; 14 ; 15 RIL 100; 16 631;

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1. .., .., .., .., .., .. // . - . 2010. . (143). . 37.

VI , , 20 11 .

2. Fernarndez-Martinez J.M., Perez-Vich B., Velasco L. Sunflower // Oil crops.

Series: Handbook of Plant Breeding / Eds. J. Vollmann, I. Rajcan. New York, 2009.

Vol. 4 P. 155232.

3. Schnabel U., Engelmann U., Horn R. Development of markers for the use of the PEF1 cytoplasm in sunflower hybrid breeding // Plant Breed. 2008. Vol. 127.

P. 587591.

4. Horn R., Kusterer B., Lazarescu E., Prufe M., Friedt W. Molecular mapping of the Rf1 gene restoring pollen fertility in PET1 based F1 hybrids in sunflower (Helianthus annuus L.) // Theor. Appl. Genet. 2003. Vol. 106. P. 599606.

5. .., .., .., .., .. //

. 2009. 6. . 69.

6. Yue B., Vick B., Cai X., Hu J. Genetic mapping for the Rf1 (fertility restoration) gene in sunflower (Helianthus annuus L.) by SSR and TRAP markers // Plant Breeding. 2010. Vol. 129. P. 2428.

7. Brunel D. A microsatellite marker of Helianthus annuus L. // Plant Mol. Biol. 1994. Vol. 24. P. 397400.

8. .., .., .. // . 2004. . 38. 2. . 1519.

9. .., .., .., .. SSR - // . 2006. 1. . 4648.

10. Saghai-Maroof M.A., Soliman K.M., Jorgensen R.A., Allard R.W. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics // Proc. Natl. Acad. Sci. USA. 1984. Vol. 81. P.

80148018.

11. .., .., .. : , , . . -: , 2010. 31 .

12. Boom R., Sol C.J.A., Salimans M.M., Jansen C.L., Wertheim-Van Dillen P.M.E., Van Der Noordaa J. Rapid and simple method for purification of nucleic acids // J. Clin. Microb. 1990. Vol. 28. 3. P. 495503.

MOLECULAR GENETIC MARKERS IN SUFLOWER BREEDING

A molecular-genetic analysis of the sunflower lines carrying the CMS and pollen fertility restorer lines was conducted. It was shown that DNA markers may be applied to evaluate inbred lines for genetic purity and to screen for economically important traits.



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